Cervical cancer bethesda classification. Variantele genice ale Interleukinei 1β -511 C>T şi neoplazia intraepitelială cervicală

Corelaţii clinice şi paraclinice în managementul neoplaziei intraepiteliale cervicale

Rotar, Florin V. Interleukin 1β is a proinflammatory cytokine that plays a critical role in chronic inflammation and carcinogenesis.

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Material and method. A case-control study was realized.

Variantele genice ale Interleukinei 1β -511 C>T şi neoplazia intraepitelială cervicală

The cases were represented by patients diagnosed with cervical intraepithelial neoplasia, positive at HPV HR testing, while controls, negative cervical cancer bethesda classification intraepithelial neoplasia and negative at HPV HR testing, were included in the control group. Chi-square statistics had a value of 0.

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Odds ration had values of 1. Designul studiului cervical cancer bethesda classification fost de tip caz-control.

Clinical and paraclinical correlations in the management of cervical intraepithelial neoplasia

Lotul de studiu a fost reprezentat de de paciente diagnosticate cu neoplazie cervicală intraepitelială şi pozitive la testarea HPV, iar lotul de control a fost reprezentat de paciente fără leziuni cervicale şi negative la testarea HPV. The mechanisms involved in this transition are extremely complex and only cervical cancer bethesda classification understood, immune signal molecules, chemokines, interleukins and the molecules involved in the control of the cellular cycle being involved 1.

Genetic particularities of these molecules could explain why fortunately only a small number of HPV Human Papilloma Virus infected women will develop cervical cancer bethesda classification cancer.

This is achieved by the excision or ablation of the squamous-cylindrical area up to the healthy tissue. In the case of persistence of high-grade lesions after repeated excision and if the patient does not want to preserve fertility, hysterectomy may be indicated. This is a surgical procedure that can be indicated for benign gynaecological pathology, preinvasive cervical neoplasia and stage IA1 of cervical cancer.

Interleukin 1b together with interleukin-1a and the antagonist of interleukin 1 belong to the interleukin 1 cytokine family, toxine in singe on a kb segment on chromosome 2. Interleukin 1b is synthesized mainly by macrophages, but also by other cells of the immune system, with cervical cancer bethesda classification roles in both acute and chronic inflammato­ry response 2.

cervical cancer bethesda classification

The fact that chronic HPV infection is a mandatory step in cervical carcinogenesis is well known 3. Previously pu­blished researches confirmed cervical cancer bethesda classification contribution of interleukin 1b in the neoplastic process by influencing tumor growth and angiogenesis 4respectively cervical cancer bethesda classification the induction of chemo resistance 5.

While performing a search throughout MEDLINE databases we have encountered articles regarding the association between this polymorphism and human pathologies 8.

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The presence of this polymorphism was found to be significantly associated with gastroduodenal ulcer 9neoplasia 10pulmonary 11 and pancreatic cancer This particular genetic polymorphism was proved to be related to gynecological and obstetrical conditions: high risk of preterm labor 16spontaneous recurrent abortions 17,18bacterial vaginosis 19polycystic ovary syndrome 20 and uterine leiomyoma The relationship between this genetic polymorphism and cervical cancer bethesda classification cancer was previously analyzed in two small studies in an Indian and respectively Korean population but not in Caucasians 22, One hundred and eleven patients with negatives results for intraepithelial lesion with negative HPV HR tests were included in the control group.

The patients were recruited from the corpus of patients hospitalized or only consulted on an ambulatory base during this period at the First Clinic of Obstetrics and Gynecology, Emergency County Hospital Cluj-Napoca, Romania, willing to participate at this study. The study was approved by the ethical committees and the written consent was obtained for each patient Biological probes collection A gynecological examination was performed for each patient.

Corelaţii clinice şi paraclinice în managementul neoplaziei intraepiteliale cervicale

A cervical cytology was obtained. Cervical cytology results were formulated according to Bethesda nomenclature Colposcopic examination was performed for each patient.

After the gynecological examination a blood sample was obtained using a 2 ml blood collection tube. The blood samples were immediately stored at °C. The analysis of cervical samples The cervical cancer bethesda classification cytology was examined by a cytologist after a preliminary Papanicolau staining in the laboratory of the above mentioned hospital.

HPV HR testing was performed using an immunohistochemistry-based kit. DNA extraction.


Genetic analysis The genomic DNA was extracted from peripheral leukocytes contained in µl of whole blood using a commercial kit Wizzard Genomic DNA Purification Kit, Promega® according to the producer instructions. The extracted DNA was stored in a freezer at °C. Briefly, the PCR reaction was done in 25 µl, total volume containing: Free nuclease water was added in order to reach the total volume.

The PCR protocol is shown in Table 2.

Genetic variants of Interleukin 1-Beta-511 C>T and cervical intraepithelial neoplasia

Table 1. Statistical analysis The quantitative continuous variables were expressed using centrality and dispersion parameters. Comparison of the distribution of genotypes on cases and controls was done using chi-square test. The differences were considered significant if p-value was lower than 0.

cervical cancer bethesda classification

CatROM program 28 was used in order to calculate the statistical parameters associated to the 2×2 contingency table for investigation of genetic polymorphisms as risk cervical cancer bethesda classification for cervical cancer. Results One hundred and twenty eight cases and one hundred and eleven controls were enrolled in this study. Each case had a diagnosis of cervical intraepithelial neoplasia based on cervical cytology.

Cervical cancer bethesda classification patients with intraepithelial neoplasia, HSIL is not the most cervical cancer bethesda classification pathology encountered 31 ; it represents the consequence of the study design. Figure 1. Categories of cervical intraepithelial neoplasia - case group Genotypes distribution is cervical cancer bethesda classification in Table 3.

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Table 3. The frequencies of IL1 β genotypes for case and control groups Genotypes distribution for each type of cervical intraepithelial neoplasia is presented in Figure 2.

Table 4.

Womens Health Pap Smear and Cervical Dysplasia

Allelic frequency for case groups and p-value associated to Z test when the frequencies on case and control groups were compared Table 5. The common allele - C - was the most frequently encountered in both groups and in all categories of cases. The results obtained in analysis on control group are as follow: C: